A Rapid, Sensitive, and Specific Detection of Aggregated α-Synuclein by a Liposome-Immobilized Cantilever Sensor
نویسندگان
چکیده
Parkinson’s disease (PD) is characterized by dopaminergic cell loss and the formation of Lewy bodies, which main component aggregated fibrillized $\alpha $ -synuclein (aSyn). Recent studies suggested that ultratrace amounts aSyn aggregates are also present in biofluid specimens, they can serve as a biomarker for PD. Because has been shown to possess prion-like property, we attempted enhance sensitivity specificity cantilever microsensor detect exploiting properties self-templating assembly lipid interaction on surface liposome-immobilized sensor. We found sensor was able successfully fibrils at very low concentration (100 pg/mL), addition monomers, were converted into presence further acted template fibrillization, lowered detection limit 10 pg/mL. The this comparable or slightly superior enzyme-linked immunosorbent assay (ELISA). Moreover, lag time significantly reduced 100–120 min, compared tens hours needed conventional ELISA, real-time quaking-induced conversion (RT-QuIC), protein misfolding cyclic amplification (PMCA) assays. Finally, preliminary measurements showed possibilities discriminating serum from PD non-PD patients. could promising tool early preclinical diagnosis
منابع مشابه
Development of a Nano-ELISA system for the rapid and sensitive detection of H9N2 avian influenza
Influenza is one of the most important viral diseases that is common among the birds and the mammalsand is caused by specific viruses that belong to the Orthomyxoviridae family. Migratory aquatic birds arethe reservoir of the disease and there is a likelihood of the disease in any region. There are differentmethods for detecting the avian influenza, but by the point of detection rates, the ELIS...
متن کاملa genre analytic study of research papers written by bilingual writers and their beliefs: a case of persian-english writers
تحقیق حاضر گزارشی است از تحلیل بخش مقدمه دو دسته از مقالات که عبارتند از: 11 مقاله از دو نویسنده دوزبانه فارسی زبان, که شامل مقدمه 4 مقاله به زبان انگلیسی و چاپ شده در مجلات بین المللی, مقدمه 3 مقاله به زبان انگلیسی و 4 مقاله به زبان فارسی چاپ شده در مجلات داخلی می شود؛ و 12 مقاله از محققان خارجی که در مجله applied linguistics به چاپ رسیده است. مبنای تئوری این تحلیل ها نظریه سوئلز (1990) یا هما...
15 صفحه اولA Sensitive Sensor for Nano-Molar Detection of 5-Fluorouracil by Modifying a Paste Sensor with Graphene Quantum Dots and an Ionic Liquid
5-fluorouracil is a widely used anticancer drug with many side effects on humans, and hence its analysis in biological samples is very important. Accordingly, a novel sensitive electrochemical approach was fabricated by incorporating graphene quantum dots (GCD) and 1-butylpyridinium bromide (BPBr) in the formulation of a carbon paste electrode (GQD/BPBr/CPE). The GQD was synthesized and charact...
متن کاملCantilever sensor for nanomechanical detection of specific protein conformations.
A fast, label-free, and multiplexed method based on piezoresistive cantilevers is reported for the detection of specific protein conformations at the nanoscale level. The ligand-binding domain of the human oestrogen receptor (ERalpha-LBD) is used as the experimental model system, and ERalpha-LBD with or without oestradiol (E2) is detected using the conformation-specific peptides alpha/betaI (Se...
متن کاملa comparison of linguistic and pragmatic knowledge: a case of iranian learners of english
در این تحقیق دانش زبانشناسی و کاربردشناسی زبان آموزان ایرانی در سطح بالای متوسط مقایسه شد. 50 دانش آموز با سابقه آموزشی مشابه از شش آموزشگاه زبان مختلف در دو آزمون دانش زبانشناسی و آزمون دانش گفتار شناسی زبان انگلیسی شرکت کردند که سوالات هر دو تست توسط محقق تهیه شده بود. همچنین در این تحقیق کارایی کتابهای آموزشی زبان در فراهم آوردن درون داد کافی برای زبان آموزان ایرانی به عنوان هدف جانبی تحقیق ...
15 صفحه اولذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: IEEE Sensors Journal
سال: 2023
ISSN: ['1558-1748', '1530-437X']
DOI: https://doi.org/10.1109/jsen.2023.3272659