A Rapid, Sensitive, and Specific Detection of Aggregated α-Synuclein by a Liposome-Immobilized Cantilever Sensor

Parkinson’s disease (PD) is characterized by dopaminergic cell loss and the formation of Lewy bodies, which main component aggregated fibrillized $\alpha $ -synuclein (aSyn). Recent studies suggested that ultratrace amounts aSyn aggregates are also present in biofluid specimens, they can serve as a biomarker for PD. Because has been shown to possess prion-like property, we attempted enhance sensitivity specificity cantilever microsensor detect exploiting properties self-templating assembly lipid interaction on surface liposome-immobilized sensor. We found sensor was able successfully fibrils at very low concentration (100 pg/mL), addition monomers, were converted into presence further acted template fibrillization, lowered detection limit 10 pg/mL. The this comparable or slightly superior enzyme-linked immunosorbent assay (ELISA). Moreover, lag time significantly reduced 100–120 min, compared tens hours needed conventional ELISA, real-time quaking-induced conversion (RT-QuIC), protein misfolding cyclic amplification (PMCA) assays. Finally, preliminary measurements showed possibilities discriminating serum from PD non-PD patients. could promising tool early preclinical diagnosis